Lihlahisoa
Trimethylol aminomethane
Sebelisa
① Bohareng ba fosfomycin, e ka boela ea sebelisoa e le vulcanization accelerator, cosmetics (cream, lotion), oli ea diminerale, parafine emulsifier, buffer ea likokoana-hloko.
② E sebelisoa e le ho monya khase ea asiti, ho lokisa li-buffer, surfactant, emulsifier le accelerator.E boetse e sebelisoa ho organic synthes.
Mokhoa oa Tlhahiso
Qhala Tris ka metsi a mouoane a mabeli (300-500ml), eketsa HCl, fetola pH ho 7.6 ka HCl (1N) kapa NaOH (1N), 'me qetellong u kenye metsi a mabeli a mouoane ho 1000ml.Mokelikeli ona ke mokelikeli oa polokelo, o bolokiloeng ka sehatsetsing ka 4 ℃.
Tlhokomeliso: Theko ea PH ea Tris-Hcl e fapana ho ea ka mocheso, kahoo e lokela ho lekanyetsoa mocheso oa kamore, kahoo liphetho tse lekantsoeng li tšepahala haholoanyane.
Sebopeho sa buffering
Tris ke setsi se fokolang se nang le pKa ea 8.1 mocheso oa kamore (25 ℃).Ho latela mohopolo oa buffering, mofuta o sebetsang oa buffering oa Tris buffers o lipakeng tsa pH7.0 le 9.2.
PH ea tharollo e nang le metsi ea Tris base e ka ba 10.5, 'me buffer ea boleng ba pH e ka fumanoa ka ho eketsa hydrochloric acid ho fetola boleng ba pH ho boleng bo lakatsehang.Leha ho le joalo, tlhokomelo e lokela ho lefshoa tšusumetsong ea mocheso ho pKa ea Tris.Digree, boleng ba PH bo theohile ka 0.03.
1M Tris-HCl 6.8 le 1.5M Tris-HCl 8.8 ke li-reagents tse sebelisoang haholo bakeng sa SDS-PAGE.
Le hoja TAE, TBE le li-reagents tse ling tse entsoeng ho tloha Tris e le li-reagents tse sebelisoang haholo bakeng sa DNA electrophoresis, TE (pH8.0) e sebelisoa haholo-holo bakeng sa ho qhibiliha ha DNA.(TE ke Tris mmoho le EDTA.)
Li-buffers tsa Tris ha li sebelisoe haholo joalo ka lihlapolli tsa li-nucleic acid le liprotheine, empa hape li na le litšebeliso tse ngata tsa bohlokoa.Tris e sebelisoa bakeng sa kholo ea kristale ea protheine tlas'a maemo a fapaneng a pH.Matla a tlase a ionic a Tris buffer a ka sebelisoa bakeng sa ho theha likhoele tse mahareng tsa lamin ho C. elegans.Tris hape ke e 'ngoe ea likarolo tsa mantlha tsa li-buffer tsa protheine electrophoresis.Ntle le moo, Tris ke setsi sa ho lokisa li-surfactants, li-accelerator tsa vulcanization, le lithethefatsi tse ling.Tris e boetse e sebelisoa e le tekanyetso ea titration.
Ka mokhoa oa litšoantšo le mongolo, e ka kenyelletsa lintlha tsa sehlahisoa, lits'ebeletso le melemo.E ka fetela ka nģ'ane ho sehlahisoa ho kenya litaba tse ling (lipelaelo tsa bareki, matšoenyeho, joalo-joalo), tšireletso ea tikoloho, lisampole tsa mahala, joalo-joalo.
1. Mehlala e lekoa ntle ho tefo ho netefatsa tšebeliso e tloaelehileng ea bareki.
2. Ho ea ka tlhokahalo ea bareki ba fapaneng, tse nyenyane ho isa ho 100g, tse kholo ho lithane tsa libarele, li ka finyella litlhoko tsa ho paka.
3. Lipehelo tse feto-fetohang tsa tefo, phetisetso ea mohala kapa kamohelo (ho fihlela litlhoko tsa kamohelo)
4. Lipalangoang tse potlakileng, letsatsing lona leo kapa letsatsing le hlahlamang li ka fanoa, ts'ebetso eohle ea ho latela tlhahisoleseding ea thepa, ho netefatsa tšebeliso e nakong ea bareki.
5. Ts'ebeletso e ntle ka mor'a ho rekisa, e nang le mamello le e hlokolosi ho rarolla mathata a mefuta eohle a ka 'nang a hlaha, joalo ka mathata a boleng, sebelisana ka botlalo le bareki ho sebetsana ka katleho le bona, u se ke ua qoba boikarabelo le karabelo e fokolang.
6. Sehlopha se setle, bokhoni bo sebetsang ba ho sebetsa le tsebo ea setsebi se etsa hore bareki ba se ke ba kholiseha feela ka lihlahisoa tsa rona, empa hape ba ikutloa hore sehlopha sa rona se ka tšeptjoa.
7. Tokelo e ikemetseng ea ho rekisa thepa ho aha 'maraka oa machabeng le tšusumetso ea lets'oao la lefats'e.
8. Ka botšepehi, lengolo le thehiloeng, lilemo tse 20 tsa histori ea khampani le botumo bo botle, lumella bareki ho phutholoha, ho rua molemo ka bobeli le ho hlōla, ho sebelisana ka botšepehi.
